Item number:	SLIM
supplier:	Guangzhou Keshite Scientific Instrument Co., Ltd
Spot status:	two months
defects liability period:	1 year
quantity	no limit
Specifications:	SLIM

Space light interference microscope
（Spatial Light Interference Microscopy，SLIM）

Space light interference microscopy technology is a new imaging technique developed in recent years, developed and patented by Dr. Gabriel Popescu, a professor of electronic and computer engineering at the University of Illinois in the United States. It can quantitatively measure all types of cells through light and ensure the accuracy of information obtained. Space light interference microscopy technology can quantify through light. The imaging method of Spatial Light Interference Microscopy (SLIM). This method can measure cell mass through two beams of light, providing a new perspective for academic debates on whether cells grow at a fixed rate or exponentially.

The sensitivity of spatial light interference microscopy technology is very high, reaching 10 μ m in mass measurement^-15The weight of micrometer sized water droplets is about 1000 flying grams. This technology can be used to measure the growth of single cells, and even changes in cellular quality; However, it is evident that its application scope will be very broad, not limited to cells. Compared with other microscopy techniques, a significant advantage of SLIM is that we can measure all types of cells - bacteria, mammalian cells, adherent cells, non adherent cells, individual cells, and cell populations - while ensuring the accuracy of the information obtained Unlike other cell imaging techniques, SLIM, as a combination of phase contrast microscopy and holographic imaging, does not require special preparation such as cell staining. Due to the fact that this technology does not require entry into cells, researchers are able to study cells in their natural state; It uses white light and can be combined with other traditional techniques, such as fluorescence, to monitor cells. We can combine more traditional methods because the new technology is an additional function of the microscope, which can use all the traditional methods while adding our technical components on top. Due to the high sensitivity of SLIM technology, researchers can monitor the situation at different stages of the cell cycle. They found that mammalian cells only showed clear exponential growth during the G2 phase (DNA synthesis phase). This discovery has significant implications not only for basic biology, but also for disease diagnosis, drug development, and tissue engineering. Can use their new technology to study different disease models. For example, they plan to use SLIM to observe the difference in growth between normal cells and cancer cells, as well as the impact of medical treatment on cell growth rates. This technology can be widely used in basic biology and clinical medical research.

Technical development team: Gabriel Popescu research group, laboratories: Department of Bioengineering, Department of Electrical and Computer Engineering, Department of Physics, Department of Cell and Developmental Biology, University of Illinois Micro Nano Technology Laboratory, Advanced Technology Research Institute, Quantitative Light Imaging Laboratory, Baylor College of Medicine, Department of Biochemistry and Molecular Biology

The optical imaging device SLIM, space light interference microscope, invented by Dr. Gabriel Popescu's research group, is a new type of microscope developed based on a patented technology called coherent controlled holographic microscopy, which can accurately complete quantitative phase imaging (QPI). This technology uses incoherent light sources (such as halogen lamps, LED lamps, etc.) to obtain high-quality quantitative phase imaging (QPI), and it is currently the only technology that can achieve quantitative phase imaging (QPI) of samples in scattering media. The unique design of SLIM makes it particularly suitable for in vitro observation experiments of live cells. SLIM has a high-end inverted microscopy technology platform, with its optical system located in a single box unit, and excellent mechanical design sufficient to meet users' many needs for experimental automation. In addition, the optical system of SLIM live cell quantitative phase microscope integrates fluorescence module, simulated DIC, and bright field imaging options, providing users with multiple optional imaging modes. The above characteristics of SLIM microscope make it a highly valuable research equipment in the fields of biology and biotechnology. Whether studying the reactions of cells after specific processing (even in highly opaque scattering media), monitoring the cell life cycle including mitosis, identifying different forms of cell death, or even analyzing cell growth, migration, morphological changes, and extracellular matrix imaging, SLIM microscopy can achieve perfect results.

Main features:

Non destructive dynamic imaging of cells
No staining or labeling required
Cell stem mass measurement
Multi mode imaging
Rich cell analysis methods
Accurate quantification of cell boundaries
Imaging in scattering media
Support long-term imaging for more than 7 days

Typical application scope:

1. Cell growth research
2. Cell dynamics research
3. Three dimensional tomographic imaging
4. Neuroscience research, brain slices, brain tissue imaging
5. Research on blood testing
6. Biomedical tissue imaging

The KOSTER&PHIOPTICS gradient optical interference microscopy system is a label free 3D quantitative tomography technique used for thick tissue samples. GLIM technology can solve the problem of multiple scattering in thick tissue samples, thereby providing high contrast sample images. This module can be installed as an external device on major brands of microscope equipment, including KOSTER microscope systems, and can be stacked with fluorescence imaging channels. It only requires an external light source and a standard C-type interface to use, which is very convenient. Guangzhou Keshite Scientific Instrument Co., Ltd. is the authorized agent of this product and can provide customization and after-sales service.The gradient light interference microscope system is an aberration free optical device that is suitable for any microscope with a 1 × video interface, including bright field, fluorescence, wide field microscopes, etc. It can be immediately transformed into a powerful 3D image platform without the need for additional accessories or microscope modifications.

brief introduction:

1. Unlike phase contrast microscopy, digital holographic microscopy is based on a unique principle of phase shift microscopy. After reflecting or passing through the surface of an object, light waves undergo phase shift due to the surface morphology of the object or the refractive index of different substances inside the object, thus carrying the three-dimensional characteristics of the object.

2. The microscope can achieve real-time presentation of three-dimensional morphology, thanks to its non scanning mechanism. The time required to capture a single hologram is determined by the camera's shutter speed, so digital holographic microscopes can easily achieve normal video rates, such as 30 frames per second.

3. Transparent samples, such as cells, can only be observed using traditional phase contrast microscopes. The transmission type digital holographic microscope records the phase shift information of light after passing through cells, which can not only observe cells, but also perform three-dimensional reconstruction and quantitative analysis. Therefore, it is also known as quantitative phase contrast microscopy. Phase shift in cells is caused by subtle changes in the refractive index of different tissues within the cell. Therefore, digital holographic microscopy observation of cells does not require any labeling, such as fluorescence staining, nanoparticles, or radiation, which will not cause any damage or external effects to the observed cells.

4. Unique optical path design, like other interference techniques, the prerequisite for digital holographic microscopy to produce interference is that the optical path difference between the two beams of light must be less than the coherence length. Due to the need to use objective lenses with different magnifications to observe objects of different sizes, the optical path of object light O will change as a result. The digital holographic microscope can automatically adjust the optical path of the reference light R according to different objective lenses, so that the optical path difference between the two beams always meets the conditions for interference. This design also achieves confocal effect under each objective lens.

5. Comparison with Confocal Microscope: Holographic quantitative phase microscope adopts non scanning technology, full field transient imaging four-dimensional measurement, single frame hologram contains three-dimensional morphology information, and the longitudinal sub nanometer measurement accuracy is determined by the intrinsic wavelength of the laser. It is easy to maintain using ordinary microscope objectives. Confocal Microscope also uses scanning technology to measure static three-dimensional morphology, and the single measurement time is long, so it cannot achieve four-dimensional morphology testing.

6. Unlabeled observation of biological cells has gained widespread attention in the field of biomedicine due to the non-invasive visualization and quantitative analysis capabilities of digital holographic microscopes for biological cells. For example, as shown in Figure 5, a digital holographic microscope can measure the three-dimensional morphology of a single red blood cell. Since it does not require scanning and the measurement process is real-time, it can also dynamically track and analyze multiple cells. The following figure shows the dynamic tracking of yeast by digital holographic microscope, which can observe the movement and cell division of yeast in real-time in three dimensions

7. Unlabeled cell imaging and analysis tools provide researchers withA groundbreaking new methodTo study the cellular morphology and dynamic behavior at the level of individual cells. They track individual cells with unparalleled stability and accuracy, without the need for labeling, and can last for hours to days without harming the cells.